Lipocalin Q83 Reveals a Dual Ligand Binding Mode with Potential Implications for the Functions of Siderocalins
- Author(s)
- Nicolas Coudevylle, Leonhard Geist, Georg Kontaxis, Markus Hartl, Klaus Bister, Robert Konrat
- Abstract
Siderocalins are particular lipocalins that participate in the innate immune response by interfering with bacterial siderophore-mediated iron uptake. Additionally, siderocalins are involved in several physiological and pathological processes such as inflammation, iron delivery, tissue differentiation, and cancer progression. Here we show that siderocalin Q83 displays an unexpected dual ligand binding mode as it can bind enterobactin and unsaturated fatty acids simultaneously. The solution structure of the siderocalin Q83 in complex with arachidonic acid and enterobactin reveals molecular details of this novel dual binding mode and the determinants of fatty acid binding specificity. Our results suggest that Q83 is a metabolic hub linking iron and fatty acid pathways. This unexpected coupling might contribute to the pleiotropic functions of siderocalins.
- Organisation(s)
- Department of Structural and Computational Biology
- External organisation(s)
- Leopold-Franzens-Universität Innsbruck
- Journal
- Biochemistry
- Volume
- 50
- Pages
- 9192-9199
- No. of pages
- 8
- ISSN
- 0006-2960
- DOI
- https://doi.org/10.1021/bi201115q
- Publication date
- 2011
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 106041 Structural biology
- Sustainable Development Goals
- SDG 3 - Good Health and Well-being
- Portal url
- https://ucrisportal.univie.ac.at/en/publications/83b5864f-e111-4b5f-8799-abc8be5b0f54