Quenched hydrogen-deuterium exchange NMR of a disease-relevant Aß(1-42) amyloid polymorph

Author(s)
M. A. Waelti, J. Orts, R. Riek
Abstract

Alzheimer’s disease is associated with the aggregation into amyloid fibrils of Aβ(1–42) and Aβ(1–40) peptides. Interestingly, these fibrils often do not obtain one single structure but rather show different morphologies, so-called polymorphs. Here, we compare quenched hydrogen-deuterium (H/D) exchange of a disease-relevant Aβ(1–42) fibril for which the 3D structure has been determined by solid-state NMR with H/D exchange previously determined on another structural polymorph. This comparison reveals secondary structural differences between the two polymorphs suggesting that the two polymorphisms can be classified as segmental polymorphs.

Organisation(s)
External organisation(s)
Eidgenössische Technische Hochschule Zürich
Journal
PLoS ONE
Volume
12
No. of pages
11
ISSN
1932-6203
DOI
https://doi.org/10.1371/journal.pone.0172862
Publication date
03-2017
Peer reviewed
Yes
Austrian Fields of Science 2012
106006 Biophysics, 106002 Biochemistry
Keywords
ASJC Scopus subject areas
General Agricultural and Biological Sciences, General, General Biochemistry,Genetics and Molecular Biology
Portal url
https://ucrisportal.univie.ac.at/en/publications/ccba117e-8ce4-4640-b2f4-ef6394316ba0