Fast NMR-Based Determination of the 3D Structure of the Binding Site of Protein–Ligand Complexes with Weak Affinity Binders
- Author(s)
- Marielle A. Wälti, Roland Riek, Julien Orts
- Abstract
In early drug discovery approaches, screening hits are often weak affinity binders that are difficult to characterize in structural detail, particularly towards obtaining the 3D structure of protein–ligand complexes at atomic resolution. NMR is the outstanding technique to tackle such problems, yet suffers from a tedious structure calculation process. NMR2 was recently developed to alleviate the laborious element of routine NMR structure calculation procedures and provides the structural information at protein–ligand interaction sites orders of magnitude faster than standard procedures. The NMR2 method was extended to weak binders and applied to the oncoproteins HDM2 and MDMX. The structure of the MDMX-SJ212 complex is reported with a Kd of approximately 0.7 μm; the complex structure of HDM2 with the mm affinity ligand #845 exhibits a new scaffold.
- Organisation(s)
- External organisation(s)
- Eidgenössische Technische Hochschule Zürich
- Journal
- Angewandte Chemie - International Edition
- Volume
- 56
- Pages
- 5208-5211
- No. of pages
- 4
- ISSN
- 1433-7851
- DOI
- https://doi.org/10.1002/anie.201612304
- Publication date
- 05-2017
- Peer reviewed
- Yes
- Austrian Fields of Science 2012
- 106006 Biophysics, 106002 Biochemistry
- Keywords
- ASJC Scopus subject areas
- Chemistry(all), Catalysis
- Portal url
- https://ucris.univie.ac.at/portal/en/publications/fast-nmrbased-determination-of-the-3d-structure-of-the-binding-site-of-proteinligand-complexes-with-weak-affinity-binders(aeb0189a-103f-4117-9878-fd1542f4901f).html